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KMID : 1005220120270040193
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Journal of Embryo Transfer
2012 Volume.27 No. 4 p.193 ~ p.203
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Factors Affecting the Development of Embryos Produced by Nuclear Transfer
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Lee Joon-Hee
Campbell Keith H. S.
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Abstract
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The development of embryos reconstructed by nuclear transfer is dependent upon numerous factors including the type of recipient cell, method of enucleation, the type of donor cell, method of reconstruction, activation, the cell cycle stage of both the donor nucleus and the recipient cytoplasm and the method of culture of the reconstructed embryos. Many of these points which have been reviewed extensively elsewhere (Sun and Moor, 1995; Colman, 1999; Oback and Wells, 2002; Renard et al., 2002; Galli et al., 2003b), here we will concentrate on main area, the production of suitable cytoplast and nuclear donor, nuclear-cytoplasmic coordination, oocyte activation, culture of reconstructed embryos, and the effects that this may have on development.
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KEYWORD
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nuclear transfer , cytoplast , nuclear donor cell , nuclear-cytoplasmic coordination , oocyte activation
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